AssayMAP Protein Sample Prep Workbench : In‑Solution Digestion: Single Plate v1.2 User Guide : App description

App description
In-Solution Digestion: Single Plate v1.2. This application enables automated digestion of 1 to 96 protein samples in a single 96-well microplate format. The In-Solution Digestion: Single Plate application is designed to maximize simplicity and flexibility for low-to-medium throughput digestion processes. The procedure consists of four successive liquid-addition steps that each transfer a reagent or mixture of reagents into a common sample plate, followed by incubation on or off deck. The sample volume, incubation time, incubation temperature, labware, and other parameters can be customized for each of the four addition steps, allowing for almost any manual in-solution digestion method to be automated on the AssayMAP Bravo Platform.
Important features
The primary features are:
Plate lidding. Enables sample and reagent plates to be introduced with microplate lids to minimize light exposure and evaporation losses.
On-deck incubation. Enables sample plate incubation after every reagent-addition step at a user-defined temperature and for a user-defined duration.
Mixed-mode pipetting. For each liquid-addition step, allows the user to choose bare AssayMAP probes or 250-µL pipette tips to transfer the liquid.
Method saving. Provides the ability to save and load custom digestion methods, to ensure absolute procedural consistency between runs.
Comparison to the In-Solution Digestion: Multi-Plate App
The following table lists the primary differences between the In-Solution Digestion: Single Plate application and the In-Solution Digestion: Multi-Plate application, which is also included in the Protein Sample Prep Workbench.