Preparing the samples

 

A small volume excess is required in all labware types to ensure proper volume transfer.

 

Make sure you use the Reagent Volume Calculator for In-Solution Digestion: Single Plate to help determine the appropriate volume required for a given experimental procedure. For details, see Using the Reagent Volume Calculator for In-Solution Digestion, Single Plate.

For a list of labware options and the corresponding excess volume recommendations, see Labware.

 

To minimize evaporation, prepare the samples immediately before run time or keep the plates lidded until the run begins.

 

When preparing the samples, you must:

•	Determine the volume of samples.

•	Transfer the samples to the microplate you want to use for the protocol run.

Creating your sample plate

Before transferring the samples, you should plan the layout of the samples in the microplate. Consider the following:

•	You can process 8 to 96 samples in parallel. The samples should be arranged in contiguous columns, therefore, the samples should be in multiples of 8.

•	The volume of samples is limited by the labware you use and the digestion reaction conditions.

To transfer the samples to an approved microplate:

1	Run the Reagent Transfer utility to transfer the samples. For instructions, see Reagent Transfer v2.1.

2	Visually inspect the sample plate. If necessary, centrifuge the sample plate to remove bubbles.

 

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