Cartridge Transfer v1.1

The Cartridge Transfer utility moves full columns of AssayMAP Cartridges from the source cartridge rack, which is a standard 96AM Cartridge Rack and Receiver Plate at deck location 6, to the destination cartridge rack, which is the 96AM Cartridge and Tip Seating Station at deck location 2.

Before you start

On the Bravo deck, make sure:

•	The empty 96AM Cartridge & Tip Seating Station is at deck location 2.

•	The 96AM Cartridge Rack and Receiver Plate is at deck location 6. It may be completely or partially filled with cartridges. Make sure you remove the lid.

•	The cartridges are arranged in full columns of eight cartridges each (if the source 96AM Cartridge Rack and Receiver Plate is partially filled).

•	The 96AM Wash Station or the later model 96 Channel Wash Station is at deck location 1.

•	All other deck locations are empty.

Accessing the Cartridge Transfer utility

To access the Cartridge Transfer utility:

In the Utility Library, locate the Cartridge Transfer utility, and then click Utility.

 

The following figure shows the interface for the Cartridge Transfer utility.

 

 

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Running the Cartridge Transfer utility

To run the utility:

1	In the A. Application Settings area, specify the following settings for the run:

 

Setting	Description
First Column in Cartridge Rack	The first available column, counting from the left side of the 96AM Cartridge Rack and Receiver Plate. The value must be between 1 and 12. This is the first column that contains cartridges or, for the reverse process, the first column to which you are returning cartridges.
Number of Columns to Transfer	The number of columns of cartridges that you want to transfer to the 96AM Cartridge and Tip Seating Station. The value must be between 1 and 12.
First Column in Seating Station	The first column, counting from the left side, of the 96AM Cartridge and Tip Seating Station to which cartridges will be transfered, or for the reverse process, from which cartridges will be transfered back to the 96AM Cartridge Rack. The value must be between 1 and 12.
Reverse Process	The option to return the moved cartridges back to their original locations. See Returning the cartridges to their original locations.

2	Confirm that the physical layout on the AssayMAP Bravo deck matches the Deck Layout and Labware Table sections of the interface.

 

Incorrect labware selections can cause a hardware collision, resulting in equipment damage. Ensure that the selections in the Labware Table exactly match the physical labware present on the Bravo deck.

 

3	Ensure that the labware are properly seated in the platepads.

 

Improperly seated labware can cause a hardware collision, resulting in equipment damage. Ensure that all labware are properly seated within the alignment features of their respective platepads.

 

4	Click Run Protocol to start the protocol run.

To monitor the progress of the run, check the Status box in the upper right corner of the form.

When the run is finished, remove the 96AM Cartridge Rack and Receiver Plate with any unused cartridges from deck location 6.

 

To stop a run in an emergency, use the hardware Emergency Stop button.

 

To pause the run, click Pause. The task currently in progress finishes before the protocol pauses. The Scheduler Paused dialog box opens. For details, see Emergency stops and pauses.

To troubleshoot errors, see the Error Recovery Guide and the Bravo Platform User Guide in the Literature Library page of the Protein Sample Prep Workbench.

 

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Returning the cartridges to their original locations

If you have accidentally transferred cartridges to the wrong columns of the 96AM Cartridge & Tip Seating Station, you can return the cartridges to their original locations in the 96AM Cartridge Rack and Receiver Plate.

To return the cartridges to their original locations:

1	Without changing any other settings from their original transfer process, Check the Reverse Process check box.

2	Click Run Protocol to reverse the original cartridge transfer process.

 

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Automation movements during the protocol

The following table shows the basic movements of the Bravo Platform when you run the Cartridge Transfer utility. Different Application Settings will alter the movements.

 

Protocol step	Head moves to deck location...	Action
Cartridge Transfer Protocol	6	Load the desired number of cartridges.
	2	Park cartridges in the Cartridge and Tip Seating Station.

 

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