Syringe Wash v2.1

The Syringe Wash v2.1 utility enables you to conduct up to 6 sequential syringe washes, using up to 6 different wash solutions (plus, the wash solution in the wash station). You select which buffer to use for each of the 6 syringe washes. The same wash buffer can be selected for multiple washes.

Before you start

 

Make sure the 96AM Cartridge & Tip Seating Station at deck location 2 is empty. (The station should not contain cartridges or pipette tips.)

 

 

The following figure shows the deck layout, and the table lists the labware options for Bravo deck locations 3–9. These labware are practical for large-volume washing operations.

 

 

Labware options	Manufacturer part number
1.2mL Deep-Well PolyPro Clear Plate	ABgene AB-1127
96-Well U-Bottom PolyPro Clear Plate	Greiner 650201
12-Column Low-Profile Reservoir	Agilent 201280-100
8-Row Low-Profile PolyPro Reservoir	Agilent 201282-100
Reservoir, Seahorse 201254-100, PP, no walls, pyramid bottom	Agilent 201254-100
Reservoir, Axygen Scientific RES-SW96-LP, 86mL pyramid bottom	Axygen Scientific RES‑SW96‑LP
96 V11 Manual Fill Reservoir	Agilent G5498B#049

Accessing the Syringe Wash utility

To access the Syringe Wash utility:

In the Utility Library, locate the Syringe Wash utility, and then click Utility.

 

The following figure shows the Syringe Wash utility interface.

 

 

Return to top

Running the Syringe Wash utility

To set up and run the Syringe Wash utility:

1	Do one of the following:

•	To create a new method, proceed to step 2.

•	To use or modify an existing method:

–	Under Select Method, click the Browse button , locate and select the method file.

–	Click Load to display all the settings associated with the selected method.

–	To use a saved method exactly as written, skip to step 7. To modify the method, proceed to step 2 to make changes.

 

2	Under Wash Settings, specify the number of Syringe Rinses:

•	Initial Syringe Washes. The number of standard Internal Syringe Washes to be conducted using the wash station at deck location 1, before starting any of the syringe washes.

•	Final Syringe Washes. The number of standard Internal Syringe Washes to be conducted using the wash station at deck location 1, after completion of all the syringe washes.

3	Set the following properties for each syringe wash. You can conduct up to six washes:

 

Property	Description
Buffer	Set the deck location of the Buffer reservoir for the given syringe wash. The same deck location can be used for multiple syringe washes. Defaults: • Wash 1: Buffer 1 (deck location 4) • Wash 2: Buffer 2 (deck location 5) • Wash 3: Buffer 3 (deck location 6) • Wash 4: Buffer 4 (deck location 7) • Wash 5: Buffer 5 (deck location 8) • Wash 6: Buffer 6 (deck location 9)
Wash Type	The type of wash: • NONE. Select this option to specify no wash. If you select None for any of the 6 syringe washes, its corresponding syringe wash will be skipped. • Cycle in Reservoir. Select this option to conduct aspirate-and-dispense cycles in the designated wash buffer location. This option will not move the wash solution out of its original location. • Discard – Waste Plate. Select this option to aspirate from the specified Wash Buffer reservoir and dispense into the Waste Plate at deck location 3. IMPORTANT Ensure that the settings you specify will not overflow the capacity of the labware designated as the Waste Plate. • Discard – Wash Station. Select this option to aspirate from the specified Wash Buffer reservoir and dispense into the wash station waste sump. Default: • Wash 1: Cycle in Reservoir • Wash 2 – Wash 6: None
Volume (µL)	The volume to be aspirated into the syringes and dispensed for each wash cycle. Range: 0–250 Default: 250
Cycles	The number of aspirate-and-dispense cycles to be conducted for each syringe wash. Range: 0–20 Default: 5
Rinse after Each Cycle	To rinse after each wash cycle in this wash, select the check box. Otherwise, clear the check box. Default: Not selected
Flow Rate (µL/sec)	The volumetric speed of the pipetting axis (w‑axis) during each wash cycle. Range: 0–300 Default: 200
Wash Cycles	The number of Internal Syringe Washes at the wash station to be conducted after all the wash cycles are completed for the given wash. Range: 0–10 Default: 0

4	In the Labware Table, select that labware you are using:

 

Incorrect labware selections can cause a hardware collision, resulting in equipment damage. Ensure that the selections in the Labware Table exactly match the physical labware present on the Bravo deck.

 

•	Deck Location 3. Select the labware for the Waste plate.

•	Deck Location 4–9. Select the labware for the Buffer at each deck location that you are using for this wash method.

Note: The wash method can include up to six syringe washes.

6	To save a new or edited method:

a	In the Save Method area, specify the new Name. If applicable, select Overwrite if Name Exists.

b	Click Save to save the settings in a method file.

 

7	Place the labware on the AssayMAP Bravo deck locations, as shown in the Deck Layout area.

8	Ensure the following:

•	The labware are properly seated in the platepads.

 

Improperly seated labware can cause a hardware collision, resulting in equipment damage. Ensure that all labware are properly seated within the alignment features of their respective platepads.

 

•	The volume of solution in the labware is sufficient for the overage requirement plus the parameters selected on the form.

For volume overage requirements for a given labware type, see the Labware Reference Guide in the Literature Library page of the Protein Sample Prep Workbench.

 

Ensure that the volume parameter specified in the form will not result in the overflow of waste at deck location 3.

 

9	Click Run Protocol to start the protocol run.

You can monitor the progress of the run in the Status box in the upper right corner of the form.

When the run is finished, remove the labware from the deck.

 

To stop a run in an emergency, use the emergency stop pendant.

 

To pause the run, click Pause. The task currently in progress finishes before the protocol pauses. The Scheduler Paused dialog box opens. For details, see Emergency stops and pauses.

To troubleshoot errors, see the Error Recovery Guide and the Bravo Platform User Guide in the Literature Library page of the Protein Sample Prep Workbench.

 

Return to top