Preparing the diluent and samples

 

To minimize evaporation, fill the Sample and Diluent plates immediately before run time or keep them covered until you run the protocol.

 

 

Incorrect labware selections can cause a hardware collision, resulting in equipment damage. Ensure that the selections in the method exactly match the physical labware present on the Bravo deck.

 

To prepare the Diluent reservoir:

Prepare the Diluent plate by putting the volume calculated by the Normalization Method Setup Tool in position A12, as this is where all the diluent will be aspirated

Note: If you are using a 12-column reservoir or an 8-row reservoir, the Total to fill Reservoir value represents the volume in the rightmost column or topmost row, respectively.

To prepare the Sample plate:

1	Ensure that the labware type for the initial Sample plate matches the labware specified in the Normalization Method Setup Tool.

If necessary, use the Regent Transfer utility to transfer the samples into the appropriate labware type. For instructions, see Reagent Transfer v2.1.

2	Ensure that the samples in the Sample plate match the volume and well positions specified in the Normalization Method Setup Tool.

3	If necessary, centrifuge the reagent labware to remove bubbles.