Before you start

This topic lists the required hardware, software, and labware for running the Reformatting utility.

Hardware

Required hardware

 

 

The AssayMAP Bravo Platform is required for running the AssayMAP protocols. The following figure and table describe the platform components.

Figure. AssayMAP Bravo Platform components

 

 

Item	Required hardware
1	Gripper upgrade
2	Bravo 96AM Head
3	96AM Wash Station
4	Pump Module 2.0 and two carboys
5	96AM Cartridge & Tip Seating Station
6	Bravo Risers, 14.6 cm
7	Peltier Thermal Station with STC controller
8	Plate Risers, 2.84 cm (two)
9	Orbital Shaking Station with Control Unit

 

 

Optional equipment

You might need the following when preparing the samples and reagents:

•	Microplate centrifuge, such as the Agilent Microplate Centrifuge or equivalent

•	Microplate sealer, such as the PlateLoc Sealer or equivalent

 

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Software

 

 

The following table lists the minimum software requirements:

 

Software	Version
Agilent VWorks Automation Control	13.1.5
Agilent Protein Sample Prep Workbench	3.2
Microsoft Excel Required for the reagent volume calculators and method setup tools.	2016

 

 

 

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Labware

 

Using different pipette tips, such as filtered tips, will result in equipment damage. Ensure that you use the standard 250-µL pipette tip (Agilent 19477-002).

 

The Reformatting protocol requires the following labware:

•	Full tip box of 96 250-µL pipette tips, Agilent 19477-002

Note: A full tip box is required for each run even if the run uses less than 96 pipette tips.

•	Labware for the source plate (deck location 2) and destination plate (deck location 6)

The following figure shows the deck locations for the labware.

Figure. Bravo deck layout (top view) for Reformatting utility

 

The following table lists the labware options. For dimensionally equivalent alternatives, well capacities, recommended overages, and other labware details, see the Labware Reference Guide in the Literature Library page of the Protein Sample Prep Workbench.

Table Labware options for source and destination plates

 

Labware	Manufacturer part number
96 ABgene 1127, 1mL Deep Well, Square Well, Round Bottom	ABgene AB-1127
96 Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt	Bio-Rad HSP‑9611
96 Costar 3363, PP Conical Bottom	Corning Costar 3363
96 Eppendorf 30129300, PCR, Full Skirt, PolyPro	Eppendorf 30129300
96 Eppendorf 96/500_V-bottom, Clear PolyPro	Eppendorf 96/500
96 Greiner 650201_U-Bottom, Clear PolyPro	Greiner 650201
96 Greiner 650207_U-Bottom, White PolyPro	Greiner 650207
96 Greiner 651201_V-Bottom, Clear PolyPro	Greiner 651201
96 Greiner 652270, PCR, Full Skirt, PolyPro	Greiner 652270
96 Greiner 675801, Half Area, Flat-Bottom, UV Star	Greiner 675801
96 Thermo Matrix 3732, V-bottom, 0.75ml Storage Tubes	Thermo Fisher Scientific 3732
96 Thermo Matrix 3735, V-bottom, 500µl Storage Tubes	Thermo Fisher Scientific 3735
96 Thermo Matrix 3744, V-bottom, 500µl ScrewTop Storage Tubes	Thermo Fisher Scientific 3744
96 Waters 186005837, Clear PolyPro	Waters 186005837
12 Column, Low Profile Reservoir, Natural PP	Agilent 201280-100
8 Row, Low Profile Reservoir, Natural PP	Agilent 201282-100

The unusable volume (also known as dead or overage volume) is the volume that cannot be reliably aspirated from the microplate well due to pipetting limitations that arise from differences in well-bottom geometry. The following table provides some general guidelines on how different microplate geometries can affect this value.

Table Guidelines on how microplate geometry can affect volume requirements

 

Well-bottom geometry	Examples	Unusable volume expectations
Flat bottom	Greiner 675801	The worst plates for minimizing dead volume. Liquids are attracted to the intersection of the plate bottom and side walls.
Round (U) bottom	Greiner 650201, Greiner 650207	Good options for minimizing dead volume. A round bottom forces liquid to collect in the center of the well.
V-bottom and PCR	• V-Bottom: Greiner 651201, Corning Costar 3363 • PCR Plate: Eppendorf 30129300, Greiner 652270	The best plates for minimizing dead volume. Steep side walls force liquid to collect in the center of the well, making it easy to access.

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