Utility description

Serial Dilution v2.0. This utility enables you to automatically generate serial dilutions in a 96‑well microplate. You create a method using the Serial Dilution Method Setup Tool. The utility uses your method to transfer the appropriate volume of diluent and source sample into the designated wells of the serial dilution plate to create the desired dilution series.

The serial dilution method specifies:

•	Whether the dilution series progresses by columns or rows and the direction of the series (such as, top to bottom, bottom to top, left to right, or right to left)

•	The number of steps in the dilution series and the number of dilution replicates (2 to 24 dilutions and 1 to 5 replicates)

•	The final volume of the serial dilution (5–500 µL)

•	If the utility will change pipette tips after every dilution step, or use the same pipette tips throughout the dilution series

•	Liquid-handling parameters, including the number of mix cycles after every dilution, whether to prewet the pipette tips, the blowout volume, and the choice of liquid class

•	If a constant volume (for example, internal standard) will be added to the dilution series after running the utility