Planning for the protocol run

Reviewing the protocol

Before you start a run, make sure you review the protocol and determine:

•	The devices used in the protocol and how to prepare them for operation. For example, you might need to load a roll of seal on the PlateLoc Sealer or install a pipette head on a Vertical Pipetting Station. See the device user guides for setup instructions.

•	The optimal device setup sequence. In general, you first set up devices that do not hold time-sensitive reagents. Leave complex preparations, which might use expensive and unstable reagents, until last.

•	The labware used in the protocol and where they should be positioned before the run starts. For example, you might have to load labware into one or more storage devices such as the Labware Stacker and the Plate Hub.

•	The waste bins that should be emptied.

•	The reservoirs that must be filled.

Be sure to check the protocol User Message tasks for setup information. If the User Message tasks prompt you to place counterweight labware, you do not have to include these steps in the setup.

Reviewing the teachpoint file

Before loading labware in storage devices, always review the teachpoint file for labware orientation information. The labware must be loaded in the same orientation as specified in the teachpoint file. In addition, load the labware such that their barcodes will not be obstructed by the robot grippers.

Related information

 

For information about...	See...
Creating or revising protocols	VWorks Automation Control User Guide
Performing dry runs	Performing dry runs
Stopping the robot in an emergency	Stopping the robot in an emergency