Calculating the Orientation threshold

About this topic

Before you start this procedure, make sure you are familiar with the workflow in Overview for setting sensor thresholds.

Procedure

To calculate the Orientation threshold:

1	At each stacker, determine the highest reading for the notch and lowest reading for the corner as follows:

a	In BenchCel Diagnostics, click the Controls tab. In the graphical display area click Stacker 1, and then click Move to sensors.

b	On the Jog/Teach tab in the Stacker Sensors area, select stack 1 from the list.

c	In the Notch Sensors area, find the highest reading for the notch and lowest reading for the corner, and then record these readings. (In the example below, the highest notch reading is 7, and the lowest corner reading is 62.)

d	Repeat step a to step c for the remaining stacks.

 

2	Find the highest notch reading (Notchhighest) among all the stacks. In the following example, the highest reading is 35 (stack 3).

 

Stack 1	Stack 2	Stack 3	Stack 4
7	25	35	15

3	Find the lowest corner reading (Cornerlowest) among all the stacks. In the following example, the lowest reading is 235 (stack 4).

 

Stack 1	Stack 2	Stack 3	Stack 4
244	238	245	235

4	Calculate the Orientation threshold as follows:

Orientation threshold = (Notchhighest + Cornerlowest)/2

In the following example, the calculated threshold is 135.

 

Highest notch reading among all stacks	35
Lowest corner reading among all stacks	235
Orientation threshold (midpoint)	135

5	On the Jog/Teach tab, type the calculated Orientation threshold.

 

After you finish setting the sensor thresholds for the specific type of labware, you can use the labware in a protocol.

Related information

 

For information about…	See…
Overall workflow for setting sensor thresholds	Overview for setting sensor thresholds
Previous steps in this workflow	• Calculating the Plate presence threshold • Determining the optimum Orientation sensor offset
Worksheet for sensor thresholds	Worksheet for setting sensor thresholds