Worksheet for setting sensor thresholds

About this worksheet

Use this worksheet to record sensor readings and facilitate your threshold calculations. See Overview for setting sensor thresholds for the procedures to calculate the thresholds.

Plate-presence threshold

Determine the Plate presence readings when microplates are unloaded

Set the initial values as follows:

•	Plate presence threshold = 225 units

•	Intensity = 100%

Record the Plate presence reading for each stack.

 

Stack 1	Stack 2	Stack 3	Stack 4	Stack 5	Stack 6

Adjust the Intensity value so that the highest Plate presence reading is less than or equal to 175, and then record the Plate presence readings for each stack.

 

Stack 1	Stack 2	Stack 3	Stack 4	Stack 5	Stack 6

Highest reading among all the stacks (Punloaded): _________________

Determine the Plate presence readings when the microplates are loaded

After loading the microplates at each stack (Load All in BenchCel Diagnostics), record the Plate presence readings for each stack.

 

Stack 1	Stack 2	Stack 3	Stack 4	Stack 5	Stack 6

Lowest reading among all the stacks (Ploaded): _________________

Calculate the Plate presence threshold

Plate presence threshold = (Punloaded + Ploaded)/2 = ______

Optimum Orientation sensor offset

Initial Orientation sensor offset

Measure A and B of your microplate.

 

Initial Orientation sensor offset = (A/2) + B = ___________ mm

Jog distance

Jog distance = Zinitial – Zadjusted = _________ mm

Adjusted Orientation sensor offset

Adjusted Orientation sensor offset =

Initial Orientation sensor offset + Jog distance = _______________ mm

Orientation threshold

In graphical display area on the Controls page, click Stacker, and then choose Move to Sensor. On the Jog/Teach tab, find the highest notch reading in the Notch Sensors area. Repeat this step for each stack.

Note: Notches have lower readings than corners.

Highest Notch Sensors readings

Record the highest notch reading for each stack.

 

Stack 1	Stack 2	Stack 3	Stack 4	Stack 5	Stack 6

Highest reading among all stacks (Notchhighest): ____________

Lowest Corner readings

Record the lowest corner reading for each stack.

 

Stack 1	Stack 2	Stack 3	Stack 4	Stack 5	Stack 6

Lowest reading among all stacks (Cornerlowest): ____________

Orientation threshold

Orientation threshold = (Notchhighest + Cornerlowest)/2 = __________