AM Cartridges Off (Bravo)

Description

The AM Cartridges Off (Bravo) () task removes the AssayMAP Bravo cartridges from the Bravo 96AM Head. The task is available only if the device profile specifies the Bravo 96AM Head. You can use the AM Cartridges Off task while the head’s syringes contain liquid, and then subsequently dispense the liquid using the syringe probes.

 

Task is available for...	Task is available in...
Bravo Platform with a gripper and a Bravo 96AM Head installed	Main Protocol, Bravo Subprocess

Task parameters

After adding the AM Cartridges Off task at the desired point in the protocol, set the following parameters in the Task Parameters area:

 

 

Parameter	Description
Location, plate	The labware involved in the AM Cartridges Off task. Ensure a cartridge rack is selected.
Location, location	The location at which the AM Cartridges Off task occurs. <auto-select> automatically places the labware at the first-available or appropriate location for the task. If accessories are installed on the deck, the software uses the accessory configuration information in Bravo Diagnostics to determine the correct location for the task.
Well selection	The well positions to use for the AM Cartridges Off task. To select fewer positions than a full head, a Set Head Mode task must precede the AM Cartridges Off task in the protocol. IMPORTANT See the Bravo Automated Liquid Handling Platform User Guide for restrictions related to using a subset of channels in the Bravo 96AM Head.

Example: Remove cartridges while the syringes contain liquid after a series of AM Aspirate tasks

Goal

After aspirating a series of reagents through the AssayMAP Bravo cartridges to bind a target to the cartridge resin bed, remove the cartridges to wash the head’s syringes before mounting the cartridges again for elution.

Implementation

In a liquid-handling subprocess, the AM Cartridges Off task is added after a series of AM Aspirate tasks and before a subsequent elute-wash subprocess.

 

Because the cartridges are removed at a cartridge rack, the Cartridge Rack is selected as the Location, plate in the Task Parameters area.

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Related information

 

For information about...	See...
Using the Bravo 96AM Head	Bravo Automated Liquid Handling Platform User Guide
AM Cartridges On task	AM Cartridges On (Bravo)
AM Aspirate task	AM Aspirate (Bravo)
AM Dispense task	AM Dispense (Bravo)
Adding devices	• Adding devices • Device user guide
Adding tasks in a protocol	Adding and deleting tasks
Set Head Mode task	Set Head Mode (Bravo)