AM Cartridges On (Bravo)

Description

The AM Cartridges On (Bravo) () task presses cartridges on a Bravo 96AM Head. The task is available only if the profile specifies the Bravo 96AM Head. You can use the AM Cartridges On (Bravo) task while the head’s syringes are empty or contain liquid.

 

Task is available for...	Task is available in...
Bravo Platform with a gripper and a Bravo 96AM Head installed	Main Protocol, Bravo Subprocess

Task parameters

After adding the AM Cartridges On task at the desired point in the protocol, set the following parameters in the Task Parameters area:

 

 

Parameter	Description
Location, plate	The labware involved in the AM Cartridges On task. Ensure a cartridge rack is selected.
Location, location	The location at which the AM Cartridges On task occurs. <auto-select> automatically places the labware at the first-available or appropriate location for the task. If accessories are installed on the deck, the software uses the accessory configuration information in Bravo Diagnostics to determine the correct location for the task.
Well selection	The well positions to use for the AM Cartridges On task. To select fewer positions than a full head, a Set Head Mode task must precede the AM Cartridges On task. IMPORTANT See the Bravo Automated Liquid Handling Platform User Guide for restrictions related to using a subset of channels in the Bravo 96AM Head.

Example: Press on cartridges in order to perform a dispense through the cartridges

Goal

Press on the cartridges while fluid is held in the Bravo 96AM Head syringes so that the fluid can be dispensed through the cartridge resin bed for collection in a sample plate.

Implementation

In a liquid-handling subprocess, an AM Aspirate task is used to aspirate elution buffer using the bare probes into the Bravo 96AM Head syringes. The AssayMAP Bravo cartridges are pressed on using the AM Cartridges On task. An AM Dispense task is used to dispense the elution buffer through the cartridges into a results plate.

 

Because the cartridges are pressed on at a cartridge rack, the Cartridge Rack is selected for the Location, plate in the Task Parameters area.

 

Related information

 

For information about...	See...
Using the Bravo 96AM Head	Bravo Automated Liquid Handling Platform User Guide
Adding tasks in a protocol	Adding and deleting tasks
AM Cartridges Off task	AM Cartridges Off (Bravo)
AM Aspirate task	AM Aspirate (Bravo)
AM Dispense task	AM Dispense (Bravo)
Set Head Mode task	Set Head Mode (Bravo)