Pipette Under Collar Begin and End (Bravo)

Description

If the assembled Vacuum Filtration Station includes the filter plate, as shown in the following figure, and you want to pipette into the filter plate that is underneath the collar, a pair of Pipette Under Collar tasks (Begin and End) are required.

•	Pipette Under Collar Begin (Bravo) (). Prepares the Bravo device for pipetting in a microplate that sits under the collar of the Vacuum Filtration Station.

•	Pipette Under Collar End (). Resets the Bravo device after pipetting under the Vacuum Filtration Station collar to prepare for subsequent tasks in the Bravo Subprocess.

 

CAUTION If you are using the collar with the blue gasket, ensure that you remove the clear gasket frame support from the top of the collar. Otherwise, a collision can occur between the pipette tips and the gasket frame support.

Figure. Vacuum Filtration Station configuration with the filter plate as part of the assembly

 

 

 

Task is available for...	Task is available in...
Bravo Platform	Main Protocol, Bravo Subprocess

IMPORTANT When the protocol runs, an error message will appear before the Pipette Under Vacuum Collar Begin task starts. The operator must click OK to proceed. Alternatively, this error can be added to the Error Library.

Task parameters

Note: The Pipette Under Collar Begin task does not have any task parameters.

Pipetting task parameter requirements

The pipetting task (for example, Dispense) that is placed between the Pipette Under Collar Begin task and Pipette Under Collar End task has the following requirements:

•	The Location, plate parameter must specify the filter plate already underneath the collar of the Vacuum Filtration Station.

Note: The Pipette Under Collar tasks are not used for configurations of the Vacuum Filtration Station that do not include the filter plate as part of the assembly.

•	The Location, location parameter must specify the actual location of the Vacuum Filtration Station. Location, location cannot be set at <auto-select>.

•	The Well selection must be for the entire plate.

 

CAUTION A collision can occur if the Bravo head is partially offset from the collar of the Vacuum Filtration Station (partial head mode) while attempting to pipette into a filter plate that is under the collar. Ensure that the well selection is for the entire plate.

Pipette Under Vacuum Collar End task parameters

 

 

Parameter	Description
Filter Plate, plate	The name of the filter plate that is underneath the collar in the assembled Vacuum Filtration Station. Select the filter plate from the list of names.
Filter Plate, location	The deck location that the filter plate occupied immediately before the Assemble Vacuum task that assembled the Vacuum Filtration Station earlier in the Bravo Subprocess. For example, if the Assemble Vacuum task took the filter plate from a Filter Plate Holder at deck location 6, the Filter Plate, location value must be set to 6.

Example

Goal

Assemble a Vacuum Filtration Station in the following configuration: Base–Collection plate–Filter plate–Collar). Aspirate liquid from a reservoir, dispense liquid into the Filter Plate under the collar. Filter for 60 seconds. Disassemble the station.

Implementation

In Bravo Diagnostics, the profile is configured such that:

•	The Vacuum Filtration Station will be assembled at deck location 3.

•	The Vacuum Filtration Station base is at deck location 3.

•	The collar is at deck location 2.

•	The Filter Plate Holder is at deck location 6.

Figure. Configuration tab in Bravo Diagnostics dialog box

 

For instructions on how to configure the device in Bravo Diagnostics, see the Bravo Automated Liquid Handling Platform User Guide.

In the protocol, the following are added, as shown:

•	Filter Plate process with a Place Plate task to place the Filter Plate at Bravo deck location 6.

•	Configured labware for the tip box, reservoir, and Collection Plate.

•	In the Bravo SubProcess, the following tasks are added: Assemble Vacuum, Tips On, Aspirate, Pipette Under Collar Begin, Dispense, Pipette Under Collar End, Toggle Vacuum, Tips Off, and Disassemble Vacuum.

Figure. Example of protocol with Pipette Under Collar tasks

 

Note the following:

•	Assemble Vacuum task Assembly order is for Base–Collection plate–Filter plate–Collar.

•	Tips On task Well selection is for the entire plate.

•	Dispense task specifies the Filter Plate that is under the collar in assembled Vacuum Filtration Station at deck location 3.

•	Pipette Under Collar Begin and End tasks bracket the Dispense task.

The following figures show some of the task parameter settings.

Figure. Assemble Vacuum task parameters

 

Figure. Tips On task parameters

 

Figure. Aspirate task parameters

 

Figure. Dispense task parameters

 

Figure. Pipette Under Collar End parameters

 

Figure. Toggle Vacuum task parameters

 

Figure. Disassemble Vacuum task parameters

 

The resulting protocol will run as follows:

1	Acknowledge that the Filter Plate is starting at deck location 6.

2	Acknowledge that the Collection Plate is starting at deck location 5.

3	Assemble the Vacuum Filtration Station (Base–Collection plate–Filter plate–Collar) as follows:

a	Move the Collection Plate from deck location 5 and place it atop the base at deck location 3.

b	Move the Filter Plate from deck location 6 and place it atop the Collection Plate at deck location 3.

c	Move the collar from deck location 2 and place it on top of the Filter Plate at deck location 3.

4	Prepare the Bravo device to pipette under the vacuum collar.

5	Dispense into the Filter Plate in the Vacuum Filtration Station at deck location 3.

6	Reset the Bravo device for tasks other than pipetting under the vacuum collar.

7	Turn on the vacuum for 30 seconds, and then turn off the vacuum.

8	Disassemble the Vacuum Filtration Station:

a	Move the collar back to deck location 2.

b	Move the Filter Plate back to deck location 6.

c	Move the Collection Plate back to deck location 5.

Related information

 

For information about...	See...
Adding tasks in a protocol	Adding and deleting tasks
Assemble Vacuum task	Assemble Vacuum (Bravo)
Disassemble Vacuum task	Disassemble Vacuum (Bravo)
Move and Filter Plate task	Move and Filter Plate (Bravo)
Toggle Vacuum task	Toggle Vacuum (Bravo, Vertical Pipetting Station)