Pump Reagent (Bravo, Vertical Pipetting Station)

Description

The Pump Reagent (Bravo) () and Pump Reagent (Vertical Pipetting Station) () tasks fill or empty the Auto Filling Reservoir and Tip Wash Station (also known as MicroWash Reservoir) by pumping for a specified number of seconds or until the percent of maximum tared weight is reached. If the reservoir is on a Weigh Station or Weigh Shelf, the pump stops fluid flow when the target weight is reached. Otherwise, gravity drain is used to empty the reservoir.

 

Task is available for...	Task is available in...
Bravo Platform	Main Protocol, Bravo Subprocess
Vertical Pipetting Station	Main Protocol, Vertical Pipetting Station Subprocess

Requirements

The following must be configured on the Bravo Platform or the Vertical Pipetting Station:

•	Autofilling Reservoir or Tip Wash Station

•	Pump Module

•	Weigh Station or Weigh Shelf (optional)

Task parameters

Note: The task parameters for Pump Reagent (Bravo) and Pump Reagent (Vertical Pipetting Station) are identical.

After adding the Pump Reagent task at the desired point in the subprocess, set the following parameters in the Task Parameters area:

 

 

 

 

Parameter	Description
Location, plate	The labware involved in the Pump Reagent task.
Location, location	The location at which the Pump Reagent task occurs. <auto-select> automatically places the labware at the first-available or appropriate location for the task. If accessories are installed on the deck or shelf, the software uses the accessory configuration information in Bravo Diagnostics or Vertical Pipetting Station Diagnostics to determine the correct location for the task.
Reservoir mode	The action of the task: • Fill • Empty
Pump speed	The speed, in percent of maximum, at which to pump the reagent.
Pump on time	The duration of time, in seconds, to run the pump: If the Use weigh station/shelf option is selected, this value specifies the maximum time (seconds) to run the pump. If the pump runs for the full duration without reaching the weigh station/shelf threshold, an error occurs.
Use weigh station/shelf	The option to use the Weigh Station or Weigh Shelf.
Weigh station/shelf action threshold	The minimum fluid weight, in percent of the full weight that was calibrated on the Weigh Station or Weigh Shelf. For example, you can set the minimum threshold at 45% so that when the fluid reaches 45% of the full weight, fluid starts to pump into the reservoir.
Weigh station stop action threshold	The maximum fluid weight, in percent of the full weight that was calibrated on the Weigh Station or Weigh Shelf. For example, you can set the stop threshold at 60% so that when the fluid reaches 60% of the full weight, fluid starts to drain or pump out of the reservoir.
Allow concurrent operation	The option to permit the accessory to operate simultaneously with other tasks.

 

 

Example: Fill the Tip Wash Station after the Tip Wash task

Goal

After some liquid-handling tasks, wash the tips, and then fill the Tip Wash Station.

Implementation

In Bravo Diagnostics, set up the Auto Filling Reservoir on a deck location. Associate the reservoir with one or more pump modules.

In the protocol, add a configured labware for the Auto Filling Reservoir in addition to the other processes and configured labware required by the protocol. In the example shown, the configured reservoir is called Wash.

In the Bravo subprocess where the liquid-handling tasks are specified, a Pump Reagent task is added after the Wash Tips task. After adding the Pump Reagent task, the task name changes to Fill or empty a reservoir in the protocol, as shown in the following example.

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In the Pump Reagents Task Parameters area, Wash (the name for the Auto Filling Reservoir) is selected, because the goal is to pump fluid into the Auto Filling Reservoir.

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Related information

 

For information about...	See...
Adding devices	• Adding devices • Device user guide
Adding tasks in a protocol	Adding and deleting tasks
Aspirate task	Aspirate (Bravo, Vertical Pipetting Station)
Dispense task	Dispense (Bravo, Vertical Pipetting Station)
Set Head Mode task	Set Head Mode (Bravo)
Tips On task	Tips On (Bravo, Vertical Pipetting Station)
Tips Off task	Tips Off (Bravo, Vertical Pipetting Station)
Wash Tips task	Wash Tips (Bravo, Vertical Pipetting Station)
Microplate-handling tasks	Setting parameters for microplate-handling tasks
Microplate-storage tasks	Setting parameters for microplate storage tasks
Scheduling tasks	Setting parameters for scheduling tasks
I/O-handling tasks	Setting parameters for I/O-handling tasks