Shake (Bravo, Vertical Pipetting Station)

Description

The Shake (Bravo) () and Shake (Vertical Pipetting Station) () tasks instructs the Orbital Shaking Station to shake.

 

Task is available for...	Task is available in...
Bravo Platform	Main Protocol, Bravo Subprocess
Vertical Pipetting Station	Main Protocol, Vertical Pipetting Station Subprocess

Requirements

To use the Shake task, you must first configure the Orbital Shaking Station in Bravo Diagnostics or Vertical Pipetting Station Diagnostics.

If you are using an Orbital Shaking Station that is connected to an Inheco STC or MTC Controller, use the Shake Plate task instead. See Shake Plate (Bravo).

Task parameters

Note: The task parameters for Shake (Bravo) and Shake (Vertical Pipetting Station) are identical.

After adding the Shake task at the desired point in the protocol, set the following parameters in the Task Parameters area:

 

 

Parameter	Description
Location, plate	The labware involved in the Shake task.
Location, location	The location at which the Shake task occurs. <auto-select> automatically places the labware at the first-available or appropriate location for the task. If accessories are installed on the deck or shelf, the software uses the accessory configuration information in Bravo Diagnostics or Vertical Pipetting Station Diagnostics to determine the correct location for the task.
Mode	The action of the task: • On. Turns on the Orbital Shaking Station. • Off. Turns off the Orbital Shaking Station. • Timed. Turns on the shaking timer. You must specify the length of time to shake. If you plan to time the shaking, add only one Shake task (the task turns on the shaking, and at the end of the time period, the shaking turns off automatically). If you are not timing the shaking, add two Shake tasks in the protocol (one to turn on the shaking and the other to turn off the shaking).
RPM	The shake speed, in revolutions per minute.
Direction	The direction to shake. Select one of the direction combinations: NWSE, NESW, NS, EW, NW/SE, NE/SW.
Time for operation in Timed mode	The length of time, in seconds, you want to leave the shaking on. At the end of the period, the shaking will turn off.
Allow concurrent operation	The option to permit the accessory to operate simultaneously with other tasks. For example, the Shake and Mix tasks can operate simultaneously. CAUTION To shake and mix concurrently, use only 96-well disposable-tip pipette heads in 96-well microplates.

Example: Shake the destination microplate after adding reagents

Goal

Shake the destination microplate after adding reagents from two source microplates.

Implementation

In the liquid-handling subprocess, a Shake task is added after the second reagent is added. In the following example, a Shake task is added in a Bravo subprocess.

 

In the Shake Task Parameters area, Destination is selected, because the goal is to shake the destination microplate.

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Related information

 

For information about...	See...
Configured labware	Planning labware use
Static labware	Planning labware use
Startup Protocol	Setting up Startup and Cleanup Protocol processes
Adding devices	• Adding devices • Device user guide
Adding tasks in a protocol	Adding and deleting tasks
Microplate-handling tasks	Setting parameters for microplate-handling tasks
Microplate-storage tasks	Setting parameters for microplate storage tasks
Scheduling tasks	Setting parameters for scheduling tasks
I/O-handling tasks	Setting parameters for I/O-handling tasks