Tips On (Bravo, Vertical Pipetting Station)

Description

The Tips On (Bravo) () and Tips On (Vertical Pipetting Station) tasks () presses disposable pipette tips on the pipette head. The task is not for use with fixed-tip pipette heads.

 

Task is available for...	Task is available in...
Bravo Platform	Main Protocol, Bravo Subprocess
Vertical Pipetting Station	Main Protocol, Vertical Pipetting Station Subprocess

Note: Options in Tips On and Tips Off tasks permit the tracking of pipette tip usage during and across protocol runs. In addition, an option in the Tips Off task allows you to reuse pipette tips for a portion of the protocol run.

Requirements

Tracking tips in serial dilution tasks

In addition to selecting the tracking option in the Tips On and Tips Off tasks, you must also turn on the tracking in the Serial Dilution Wizard. For more detailed information, see Serial Dilution (Bravo, Vertical Pipetting Station).

Tracking tips across different protocols

If you want to track pipette tip usage across different protocols that contain Bravo and Vertical Pipetting Station Subprocesses, make sure:

•	The protocols reference the same device file.

•	The tip boxes are at the same physical deck locations.

•	In the software, the tip boxes are configured at the same locations across the protocols.

Note: When a set of tip boxes are designated as a process plate, tip usage is tracked during the protocol run. At the end of a run, the software resets the tip box to the original state. The software assumes that in each subsequent run, you will load tip boxes in the original state before the run.

Task parameters

Note: The task parameters for Tips On (Bravo) and Tips On (Vertical Pipetting Station) are similar.

After adding the Tips On task at the desired point in the protocol, set the following parameters in the Task Parameters area:

 

 

Parameter	Description
Location, plate	The labware involved in the Tips On task.
Location, location	The location at which the Tips On task occurs. <auto-select> automatically places the labware at the first-available or appropriate location for the task. If accessories are installed on the deck or shelf, the software uses the accessory configuration information in Bravo Diagnostics or Vertical Pipetting Station Diagnostics to determine the correct location for the task.
Allow automatic tracking of tip usage	The option to allow the software to track pipette tip usage during the protocol run or across different protocol runs. When you start the run, the software will determine the positions to use in the tipbox. If you select the option in this task, you must also select the option in the Tips Off task. If you do not select this option, you must specify the positions to use in the tip box using the Well selection parameter.
Well selection	The well positions to use for the Tips On task. This parameter is available only for manual tracking of pipette tips.

Example: Press tips on before liquid-handling tasks on the Bravo Platform

Goal

Make sure tips are pressed on before liquid-handling tasks start.

Implementation

In the liquid-handling subprocess, a Tips On task is added at the beginning. In the following example, because the protocol is run on a Bravo Platform, a Set Head Mode task must be added before the Tips On task.

 

Related information

 

For information about...	See...
Adding devices	• Adding devices • Device user guide
Adding tasks in a protocol	Adding and deleting tasks
Tips On task	Tips On (Bravo, Vertical Pipetting Station)
Serial Dilution task	Serial Dilution (Bravo, Vertical Pipetting Station)
Set Head Mode task	Set Head Mode (Bravo)
Microplate-handling tasks	Setting parameters for microplate-handling tasks
Microplate-storage tasks	Setting parameters for microplate storage tasks
Scheduling tasks	Setting parameters for scheduling tasks
I/O-handling tasks	Setting parameters for I/O-handling tasks