Creating and editing pipetting techniques

Creating a pipette technique

You can create a Pipette Technique in two ways:

•	When setting task parameters in a protocol

•	Using the Tools menu

To create a Pipette Technique when setting task parameters:

1	In the VWorks window, create a new protocol.

2	Add the Subprocess (Bravo) or Subprocess (Vertical Pipetting Station) task.

3	Add one of the following tasks:

•

Aspirate

•

Dispense

•

Mix

•

Pin Tool

4	In the Task Parameters area, select Edit technique in the Pipette Technique property list.

 

The Pipette Technique Editor dialog box opens.

 

5	Click Create new technique, type a technique name, and then click OK. The new technique name appears in the Pipette Technique Editor dialog box. In addition, the X/Y Offset parameters appear to the right of the technique name.

 

6	In the East/west offset (-100 - 100%) box, type the distance (in percent of well radius) you want the pipette to move in the X direction:

•	0 does not move the pipette horizontally.

•	Positive value moves the pipette to the right.

•	Negative value moves the pipette to the left.

Note: The pipette offset directions do not correspond to the robot jog directions in Bravo and Vertical Pipetting Station Diagnostics.

 

7	In the North/south offset (-100 - 100%) box, type the distance (in percent of well radius) you want the pipette to move in the Y direction.

•	0 does not move the pipette forward or backward.

•	Positive value moves the pipette backward.

•	Negative value moves the pipette forward.

Note: The pipette offset directions do not correspond to the robot jog directions in Bravo and Vertical Pipetting Station Diagnostics.

 

8	When you are finished, click Update the selected technique.

To create a Pipette Technique using the Tools menu:

1	In the VWorks window, on the Tools menu, click Pipette Technique Editor. The Pipette Technique Editor dialog box opens.

2	Click Create new technique, type a technique name, and then click OK. The new technique name appears in the Pipette Technique Editor dialog box. In addition, the X/Y Offset parameters appear to the right of the technique name.

3	Set the parameters in the X/Y Offset Pipetting table.

4	When you are finished, click Update the selected technique.

Editing a pipette technique

You can edit a Pipette Technique in two ways:

•	When setting task parameters in a protocol

•	Using the Tools menu

To edit a Pipette Technique when setting task parameters:

1	In the VWorks window, open the protocol.

2	In the Main Protocol area, select the task for which the Pipette Technique will change.

3	In the Task Parameters area, select Edit technique in the Pipette Technique property list.

 

The Pipette Technique Editor dialog box opens.

 

4	Select the Pipette Technique on the left side of the dialog box.

5	Make the desired changes in the X/Y Offset Pipetting table.

6	When you are finished, click Update selected technique.

To edit a Pipette Technique using the Tools menu:

1	In the VWorks window, on the Tools menu, click Pipette Technique Editor. The Pipette Technique Editor dialog box opens.

2	Select the Pipette Technique on the left side of the dialog box.

3	Make the desired changes in the X/Y Offset Pipetting table.

4	When you are finished, click Update selected technique.

Related information

 

For information about...	See...
Pipette Techniques	About the Pipette Technique Editor
Managing Pipette Techniques	Managing pipetting techniques
Pipette Technique files	Storing Pipette Technique files
Liquid-handling tasks	Setting parameters for liquid-handling tasks