Planning the cartridge layout
Before transferring the reagents, you should plan the layout of the cartridges in the microplate. Consider the following:
• You can process 1 to 96 AssayMAP Fe(III)-NTA cartridges in parallel. The position of the cartridges in the seating station dictates the position of the reagent and buffer solutions in the microplates and reservoirs.
• If you have fewer than 96 Fe(III)-NTA cartridges, make sure the cartridges occupy full columns in the seating station, as the figure below shows.
The default protocol settings assume that cartridges will be arranged in multiples of 8 in a column-based configuration. Also, the AssayMAP Bravo Platform applies differential pressure to seat cartridges based on the number of full columns of cartridges. To achieve proper cartridge seating, entire columns must be used.
• If the number of Fe(III)-NTA cartridges you have is not a multiple of 8, use AssayMAP Resin-Free cartridges to fill the empty well positions. This will prevent liquids from dripping on the deck or being dispensed on the deck during the Cup Wash steps.
Figure Examples of cartridge and reservoir layout: A) Multiple of 8 Fe(III)-NTA cartridges, B) Fe(III)-NTA cartridges and Resin-Free cartridges
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