About the Workflow Library
The Workflow Library is a collection of protein sample preparation workflows. Each workflow consists of a set of applications that are performed in sequence to complete an experiment. In addition, the workbench contains links to utilities that facilitate sample and reagent preparation, the transition between applications, and the transition between the output of the final application and the input of the analytical instrument.
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General Workflows
Workflow | Enables the … |
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Affinity Purification | Creation of custom affinity cartridges that can be used to enrich target molecules. The workflow consists of the following applications: • Immobilization. For details, see the Immobilization v3.0 User Guide. • Affinity Purification. For details, see the Affinity Purification v3.0 User Guide. |
Peptide Sample Prep | Digestion of proteins, the desalting of the peptide resulting from the digestion, and optionally the fractionation of the peptide mixture. The workflow consists of the following applications: • In-Solution Digestion. For details, see the In-Solution Digestion: Single Plate v2.0 User Guide. • Peptide Cleanup. For details, see the Peptide Cleanup v4.0 User Guide. • Fractionation. For details, see the Fractionation v2.0 User Guide. |
Rapid Antibody Digestion | Desalting of denatured antibodies, and the rapid digestion of these antibodies. The workflow consists of the following applications: • Affinity Purification. For details, see the Affinity Purification v3.0 User Guide. • In-Solution Digestion. For details, see theIn-Solution Digestion: Single Plate v2.0 User Guide. • Protein Cleanup. For details, see the Protein Cleanup v3.0 User Guide. |
Post-Translational Modification Workflows
Workflow | Enables the … |
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Phosphopeptide Enrichment | Digestion of proteins, the desalting of the peptide resulting from the digestion, and the enrichment of the phosphopeptides. The workflow consists of the following applications: • In-Solution Digestion. For details, see the In-Solution Digestion. For details, see the In-Solution Digestion: Single Plate v2.0 User Guide. • Peptide Cleanup. For details, see the Peptide Cleanup v4.0 User Guide. • Phosphopeptide Enrichment. For details, see the Phosphopeptide Enrichment v3.0 User Guide. |
Evosep Workflow
Workflow | Enables the … |
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Evosep 96AM Workflow | Automation of the pipetting steps for the Evotip Pure loading protocol. For details, see the Evosep - 96AM Workflow Quick Start Guide at Quick start guides. |