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Application | Automates the … | See… |
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Affinity Purification v3.0 | Affinity purification of target molecules using AssayMAP cartridges. This is the recommended Affinity Purification application. | |
Affinity Purification: Aspiration Mode v3.0 | Affinity purification of target molecules using AssayMAP cartridges. The sample load and wash steps in this protocol are drawn into rather than pushed through the cartridges, which is in contrast to the Affinity Purification v3.0 protocol. This application may be useful for certain workflows, but is more prone to clogging. | |
Fractionation v2.0 | Stepwise fractionation of samples from AssayMAP cartridges in up to six fractions using buffers with increasing ionic strength, increasing pH, or increasing organic concentration. | |
IMAC Cartridge Customization v2.0 | Stripping and charging of AssayMAP Fe(III)-NTA cartridges with the desired metal of choice for immobilized metal affinity chromatography (IMAC) experiments. | |
Immobilization v3.0 | Immobilization of antibodies and other ligands to PAW, PGW, or SAW cartridges. | |
In-Solution Digestion: Single Plate v2.0 | Digestion of 8 to 96 protein samples in a 96-well microplate. The protocol includes five generic liquid-handling steps that successively transfer a reagent from a reagent plate at deck location 5, 6, 7, 8, or 9, into the Sample Plate at deck location 4. | |
In-Solution Digestion: Multi-Plate v2.0 | Digestion of 8 to 384 protein samples in up to four 96-well microplates in a single run. | |
On-Cartridge Reaction v2.0 | Reactions on AssayMAP cartridges that contain immobilized target molecules. | |
Peptide Cleanup v4.0 | Cleanup of peptide samples using AssayMAP cartridges. This is the recommended Peptide Cleanup application. | |
Peptide Cleanup: Aspiration Mode v3.0 | Cleanup of peptide samples using AssayMAP cartridges. The sample load and wash steps in this protocol are drawn into rather than pushed through the cartridges, which is in contrast to the Peptide Cleanup v4.0 protocol. This application may be useful for certain workflows, but is more prone to clogging. | |
Phosphopeptide Enrichment v3.0 | Phosphopeptide enrichment using the AssayMAP Fe(III)-NTA or TiO2 cartridges. | |
Protein Cleanup v3.0 | Cleanup of protein samples using the AssayMAP RP-W cartridges. |