App description
In-Solution Digestion: Single Plate v2.0. This application enables automated in-solution reactions of 1 to 96 samples in a single 96-well microplate format. The In-Solution Digestion: Single Plate application is designed to maximize simplicity and flexibility for low-to-medium throughput reactions. The procedure consists of five successive liquid-addition steps that each transfer a reagent or mixture of reagents into a common sample plate, followed by incubation on or off deck. The sample volume, incubation duration, incubation temperature, labware, and other parameters can be customized for each of the five addition steps, allowing for almost any manual in-solution reaction method to be automated on the AssayMAP Bravo Platform.
Primary features
The primary features are:
• Plate lidding. Enables sample and reagent plates to be introduced with microplate lids to minimize light exposure and evaporation losses.
• On-deck incubation. Enables timed sample plate incubation after every reagent-addition step at a user-defined temperature and for a user-defined duration.
• Mixed-mode pipetting. For each liquid-addition step, allows the user to choose bare AssayMAP probes or 250-µL pipette tips to transfer the liquid.
• Method saving. Enables creating methods for future use. A generic standard in-solution protein digestion method is included that you can use for initial testing purposes or as a template for creating custom digestion processes.
• Experiment ID database. Captures the steps executed and the settings used during each run of an application or utility.
Comparison to In-Solution Digestion: Multi-Plate
The following table lists the primary differences between the In-Solution Digestion: Single Plate application and the In-Solution Digestion: Multi-Plate application, which is also included in the Protein Sample Prep Workbench.
Feature | In-Solution Digestion: Single Plate | In-Solution Digestion: Multi-Plate |
|---|---|---|
Throughput | 8 to 96 samples per run (1 full plate) | 8 to 384 samples per run (up to 4 full plates) |
Digestion method | Flexible You can define any digestion method (up to 5 liquid-addition steps per run). | Fixed Specifically designed to run the following: Denature/Reduce > Alkylate > Dilute > Digest. |
Labware options | Variable You may select labware for sample and reagent plates from a pre-approved list. | Fixed You must use specific labware for every reagent and sample plate. |
Pipetting options | For each addition step, you may use pipette tips or the bare probes. | You may use only the bare probes. |