Performing a task using Bravo Diagnostics

About this topic

You can use Bravo Diagnostics to perform tasks, such as Tips On, Aspirate, Mix, and Dispense. Performing these tasks in Bravo Diagnostics can be useful for development and troubleshooting purposes.

 

The red Stop motors button in Bravo Diagnostics does not perform an immediate stop. The Bravo head can continue to move in the same direction at the same speed after you click the button. To perform an emergency stop, press the red button on the emergency-stop pendant.

 

Before you start

Do the following:

•	Initialize the profile in Bravo Diagnostics, and then click the Processes tab.

•	Verify that the correct labware is positioned on the Bravo deck.

 

Procedure

Performing a task requires that you:

•	Choose a location

•	Select a task and task parameters

•	Select labware

To perform a task:

1	Select a location. You can do one of the following:

•	Click the location in the graphical display.

•	Select from the Location list.

2	Select the labware from the Labware at selected location list.

3	If the task involves two locations, repeat step 1 and step 2.

For example, if you select the Assemble Vacuum task for a Vacuum Filtration Station, you could have a collection plate at Location 1 and a filter plate at Location 2.

4	Series III disposable-tip heads and Bravo 96AM Head only. To use one row or column of channels instead of all channels in the head:

a	Click Set head mode.

b	In the Head Mode Selector dialog box, select the barrels, and then click OK.

 

Note: If you select a single row on a disposable-tip head, make sure you retract the head stripper pins.

5	If you are using the head in serial mode or the head has fewer tips than the plate has wells, select the quadrant or quadrants in the Well Selection and Head Mode area.

Click a representative well in the plate graphic to select the corresponding quadrant of wells. The selection message appears below the plate graphic.

6	In the Command Parameters area:

a	Select the process from the Command to execute list.

b	Set the parameter values for the command.

 

Command to execute	Parameter description
Aspirate	Aspirate task parameters
Assemble Vacuum Disassemble Vacuum	Vacuum Filtration Station task parameters
Dispense	Dispense task parameters
Dispense to Waste	Dispense to Waste task parameters
Mix	Mix task parameters
Pump Reagent	Pump Reagent parameters
Shake	Shake task parameters
Tips On Tips Off	No parameter settings
Move and Filter Plate	Vacuum Filtration Station task parameters
Wash Tips	Wash Tips task parameters
Pin Tool	Pin Tool task parameters
Bravo 96AM Head only
AM Aspirate	AM Aspirate task parameters
AM Cartridges On AM Cartridges Off	No parameter settings
AM Dispense	AM Dispense task parameters
AM Mix	AM Mix task parameters
AM Wash Syringes

c	To start the process, click Execute command.

To stop a process before completion, click Stop motors to stop the movement of the Bravo head. To re-enable the motors, click Enable all motors on the Jog/Teach tab.

Alternatively, you may click the emergency-stop button on the pendant to stop the diagnostic process.

Related information

 

For information about...	See...
Bravo task parameters	Processes tab quick reference
Setting up the Bravo Platform	Workflow for setting up the Bravo Platform