Before you start
This topic lists the required hardware, software, and labware for running the In‑Solution Digestion protocol. If you have questions about these items, contact Agilent Customer Service.
Hardware
The following figure and table show the components of the AssayMAP Bravo Platform, which is required for running the AssayMAP protocols.
Figure AssayMAP Bravo Platform components
 |
Item | Required hardware |
|---|---|
1 | Gripper upgrade |
2 | Bravo 96AM Head |
3 | 96AM Wash Station |
4 | Pump Module 2.0 and two carboys |
5 | 96AM Cartridge & Tip Seating Station |
6 | Risers, 146 mm |
7 | Peltier Thermal Station with STC controller |
8 | Custom plate nest |
9 | Thermal plate insert for efficient heat transfer on the Peltier Thermal Station |
10 | Orbital Shaking Station with Control Unit |
Optional equipment. The following equipment is recommended when preparing the samples and reagents:
• Microplate centrifuge, such as the Agilent Microplate Centrifuge or equivalent
• Microplate sealer, such as the Agilent PlateLoc Thermal Microplate Sealer or equivalent
• Heated Incubator
Software
The following table lists the minimum software requirements.
Software | Version |
|---|---|
Agilent VWorks Plus (compliance-enabled edition) or VWorks Standard | 14.4 |
Agilent Protein Sample Prep Workbench | 4.0.2 |
Microsoft Excel Required for the reagent volume calculators and method setup tools. | Microsoft Office 365 32-bit edition |
For an overview of the software components, see Overview of software architecture.
Labware
The In-Solution Digestion: Single Plate application does not use AssayMAP cartridges. All pipetting operations are handled in 96-well microplates using either pipette tips mounted on the AssayMAP probes or the bare AssayMAP probes.
Note: Pipette tips are required for addition steps that include a deep-well plate on the AssayMAP Bravo Platform.
The following table provides a complete list of labware options and the corresponding deck locations.
The following figure shows the nine Bravo deck locations for labware.
Use only the labware specified for each deck location. Using different labware or placing labware at unapproved deck locations can cause a collision resulting in equipment damage.Figure Labware locations on the Bravo deck (top view)
 |
Labware | Manufacturer part number* | Deck location options |
|---|---|---|
250-µL pipette tips (preloaded in 96AM Cartridge & Tip Seating Station, Agilent G5409-20025) | Agilent 19477-002 | 2 |
Lid, Universal** | Agilent 200858-100 | 4—9 |
96 Eppendorf 30129300, PCR, Full Skirt, PolyPro | Eppendorf 30129300 | 4—9 |
96 Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt | Bio-Rad HSP‑9611 | 4—9 |
96 Greiner 652270, PCR, Full Skirt, PolyPro | Greiner 652270 | 4—9 |
96 Greiner 650201_U-Bottom, Clear PolyPro | Greiner 650201 | 4—9 |
96 Greiner 650207_U-Bottom, White PolyPro | Greiner 650207 | 4—9 |
96 Greiner 651201_V-Bottom, Clear PolyPro | Greiner 651201 | 4—9 |
96 Costar 3363, PP Conical Bottom | Corning Costar 3363 | 4—9 |
96 ABgene 1127, 1mL Deep Well, Square Well, Round Bottom | ABgene AB-1127 | 4—9 |
96 Eppendorf 96-500_V-bottom, Clear PolyPro | Eppendorf 96/500 | 4—9 |
96 Eppendorf 96-1000_U-bottom, Clear PolyPro | Eppendorf 96/1000 | 4—9 |
96 Waters 186005837, Clear PolyPro | Waters 186005837 | 4—9 |
12 Column, Low Profile Reservoir, Natural PP | Agilent 201280-100 | 5—9 |
8 Row, Low Profile Reservoir, Natural PP | Agilent 201282-100 | 5—9 |
Reservoir, Seahorse 201254-100, PP, 1 Well, pyramid bottom | Agilent 201254-100 | 5—9 |
Reservoir, Axygen Scientific RES‑SW96-LP, 86mL pyramid bottom | Axygen Scientific RES‑SW96-LP | 5—9 |
96 V11 Manual Fill Reservoir | Agilent G5498B#049 | 5—9 |
96 Red PCR Insert + Eppendorf 30129300, PCR, Full Skirt | Agilent insert (provided) and Eppendorf 30129300 | 4 |
96 Red PCR Insert + Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt | Agilent insert (provided) and Bio-Rad HSP‑9611 | 4 |
96 Red PCR Insert + Greiner 652270, PCR, Full Skirt, PolyPro | Agilent insert (provided) and Greiner 652270 | 4 |
96 Greiner U-Bottom Thermal Insert + Greiner 650201_U-Bottom, Clear PolyPro | Agilent G5498B#126 and Greiner 650201 | 4 |
96 Greiner V-Bottom Thermal Insert + Greiner 651201_V-Bottom, Clear PolyPro | Agilent G5498B#126 and Greiner 651201 | 4 |
96 AbGene U-Bottom Thermal Insert + AbGene 1127, Square Well, Round Bottom | Agilent G5498B#127 and ABgene AB-1127 | 4 |
*For dimensionally equivalent alternatives and other labware details, see the Labware Reference Guide in the Literature Library page of the Protein Sample Prep Workbench. **Lidded plates are allowed at these deck locations, not the lid by itself. If you plan to use a lidded plate, ensure that you use only the approved lid type. | ||
Reagents
The types and amounts of reagents required to prepare for the In-Solution Digestion protocol depends on a combination of factors, including specific chemistry requirements, the number of samples to process, and volumes and concentrations of reagents necessary to conduct your digestion process. The Regent Volume Calculator for In-Solution Digestion calculates the reagent volumes required including the overages.
All labware require volume overage for the protocol to execute properly. Use the Reagent Volume Calculator to determine volume requirements for specific protocol conditions. For more information, see Preparing the solutions.