BenchCel User Guide : Setting sensor thresholds : Worksheet for setting sensor thresholds
Worksheet for setting sensor thresholds
About this worksheet
Use this worksheet to record sensor readings and facilitate your threshold calculations. See Overview for setting sensor thresholds for the procedures to calculate the thresholds.
Plate-presence threshold
Determine the Plate presence readings when microplates are unloaded
Set the initial values as follows:
Plate presence threshold = 225 units
Intensity = 100%
Record the Plate presence reading for each stack.
 
Stack 1
Stack 2
Stack 3
Stack 4
Stack 5
Stack 6
 
 
 
 
 
 
Adjust the Intensity value so that the highest Plate presence reading is less than or equal to 175, and then record the Plate presence readings for each stack.
 
Stack 1
Stack 2
Stack 3
Stack 4
Stack 5
Stack 6
 
 
 
 
 
 
Highest reading among all the stacks (Punloaded): _________________
Determine the Plate presence readings when the microplates are loaded
After loading the microplates at each stack (Load All in BenchCel Diagnostics), record the Plate presence readings for each stack.
 
Stack 1
Stack 2
Stack 3
Stack 4
Stack 5
Stack 6
 
 
 
 
 
 
Lowest reading among all the stacks (Ploaded): _________________
Calculate the Plate presence threshold
Plate presence threshold = (Punloaded + Ploaded)/2 = ______
Optimum Orientation sensor offset
Initial Orientation sensor offset
Measure A and B of your microplate.
 
Initial Orientation sensor offset = (A/2) + B = ___________ mm
Jog distance
Jog distance = Zinitial – Zadjusted = _________ mm
Adjusted Orientation sensor offset
Adjusted Orientation sensor offset =
Initial Orientation sensor offset + Jog distance = _______________ mm
Orientation threshold
In graphical display area on the Controls page, click Stacker, and then choose Move to Sensor. On the Jog/Teach tab, find the highest notch reading in the Notch Sensors area. Repeat this step for each stack.
Note: Notches have lower readings than corners.
Highest Notch Sensors readings
Record the highest notch reading for each stack.
 
Stack 1
Stack 2
Stack 3
Stack 4
Stack 5
Stack 6
 
 
 
 
 
 
Highest reading among all stacks (Notchhighest): ____________
Lowest Corner readings
Record the lowest corner reading for each stack.
 
Stack 1
Stack 2
Stack 3
Stack 4
Stack 5
Stack 6
 
 
 
 
 
 
Lowest reading among all stacks (Cornerlowest): ____________
Orientation threshold
Orientation threshold = (Notchhighest + Cornerlowest)/2 = __________