Preparing the diluent and samples
Before you start
If you have not already done so, display the method’s labware selections and diluent and sample preparation instructions by doing one of the following:
• Open the Normalization utility and select the method. For details, see Setting up the protocol.
• Export the method and view it in Microsoft Excel. For details, see Exporting and importing AssayMAP methods.
To minimize evaporation, fill the Sample and Diluent plates immediately before run time or keep them covered until you run the protocol.
Incorrect labware selections can cause a hardware collision, resulting in equipment damage. Ensure that the selections in the method exactly match the physical labware present on the Bravo deck.Preparing the Diluent reservoir
To prepare the Diluent reservoir:
Prepare the Diluent plate by putting the volume calculated by the Normalization Method Setup Tool in position A12. All the diluent will be aspirated from this location.
Note: If you are using a 12-column reservoir or an 8-row reservoir, the Total to fill Reservoir value represents the volume in the rightmost column or topmost row, respectively.
Transferring samples to the microplate
To transfer the samples to the microplate:
1 Ensure that the labware type for the initial samples plate matches the labware specified in the Normalization Method Setup Tool.
2 Run the Reagent Transfer utility or Reformatting utility to transfer the samples. For instructions, see one of the following:
Ensure that the volume and well positions are as specified in the Normalization Method Setup Tool.
3 If necessary, centrifuge the sample labware to remove bubbles.