Setting tip and well properties for pipetting
If the VWorks software is controlling a Bravo Platform, you must set the properties for the labware type in the Pipette/Well Definition sub-tab.
Before you start
• You must be logged in as an administrator or technician to perform this procedure.
• You must first create an entry for the labware.
• VWorks Plus only. Each labware entry is a record of interest that transitions through development states. A VWorks technician or administrator can edit labware entries that are In Development or In Validation. The parameter settings in the Labware Entries tab are unavailable if the labware entry is Released.
Setting properties
To set pipette/well properties:
1 Open the Labware Editor.
2 In the Labware Entries tab, click the Pipette/Well Definition sub-tab.
Figure Pipette/Well Definition sub-tab
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3 Enter the values for the available parameters according to the labware type that you are defining. The following table provides a description of each parameter.
Use calipers to carefully measure the labware you are defining in the Labware Editor.Parameter | Description |
|---|---|
Well volume | Maximum volume (µL) of fluid for one well. |
Well depth | Distance (mm) from the top of the microplate to the bottom of the well. |
Well diameter | Diameter of the well (mm). |
Well Geometry | Shape of the wells: round or square. |
Well-Bottom Shape | Shape of the well bottoms: rounded, flat, or V‑shaped. |
Row-wise teachpoint to well | Distance (mm) from the teachpoint to the center of the A1 well along the row (letter axis). This setting should be 0 mm for standard 96-well microplates and 2.25 mm for standard 384-well microplates. |
Column-wise teachpoint to well | Distance (mm) from the teachpoint to the center of the A1 well along the column (number axis). This setting should be 0 mm for standard 96-well microplates and 2.25 mm for standard 384-well microplates. |
Row-wise well to well | Distance (mm) from well-center to well-center across the row. This setting should be 9 mm for standard 96-well microplates and 4.5 mm for standard 384-well microplates. |
Column-wise well to well | Distance (mm) from well-center to well-center across the column. This setting should be 9 mm for standard 96-well microplates and 4.5 mm for standard 384-well microplates. |
Disposable tip capacity | Volume capacity (µL) of the disposable tips when labware is a tip box. |
Disposable tip length | Length (mm) of the disposable tips being used when labware is a tip box. |
Related information
For information about... | See... |
|---|---|
Workflow for adding labware to the Labware Editor | |
Opening the Labware Editor | |
Setting general properties for labware | |
Setting microplate properties for labware |