Dispense to Waste (Agilent Bravo)
Description
The Dispense to Waste (Agilent Bravo) (
) task moves the pipettes by a horizontal offset and then dispenses used fluid in between the chimneys in the MicroWash Reservoir. The software calculates the horizontal offset automatically based on the labware definition for the MicroWash Reservoir (also known as the Tip Wash Station).
) task moves the pipettes by a horizontal offset and then dispenses used fluid in between the chimneys in the MicroWash Reservoir. The software calculates the horizontal offset automatically based on the labware definition for the MicroWash Reservoir (also known as the Tip Wash Station).Task is available for... | Task is available in... |
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MicroWash Reservoir (Tip Wash Station) on Bravo Platform | Main Protocol, Bravo Subprocess |
Task parameters
After adding the Dispense to Waste task at the desired point in the protocol, set the following parameters in the Task Parameters area:
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Parameter | Description |
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Location, plate | The wash station involved in the Dispense to Waste task. |
Location, location | The location of the wash station at which the Dispense to Waste task occurs. <auto-select> If accessories are installed on the deck, the software uses the accessory configuration information in Bravo Diagnostics to determine the correct location for the task. |
Empty tips | The option to empty all the liquid from the head into the wash station outside the chimneys. |
Volume (0–250 µL) | The volume of liquid to be dispensed from each pipette. |
Blowout volume (0–250 µL) | Specifies the volume of air to dispense after the main volume has been dispensed while the tips are still in the wash station. |
Liquid class | The pipetting speed and accuracy.  To ensure consistent pipetting, always select a liquid class for liquid-handling tasks. |
Override dispense flow rate from liquid class | The option to override the dispense velocity specified in the liquid class. Selecting this option enables you to specify a value for the dispense flow rate without changing the liquid class. |
Dispense flow rate (0–500 µL/s) | The numerical value or the JavaScript variable that will override the dispense velocity setting in the liquid class. A JavaScript variable enables the value to be assigned later, for example through a VWorks form. Using a VWorks form, an operator could easily change the flow rate for a dispense step in increments from as low as 1 µL/min up to 1001 µL/min using the same liquid class. If the task is included in a VWorks macro, a JavaScript variable enables you to change the value for the task at the macro level. The software requires that the flow rate value be in microliters per second (µL/s) at run time. If you want an operator to enter the value in microliters per minute (µL/min), you can use scripting to convert the values for the software to use. |
Distance from well bottom (mm) | The absolute distance between the end of the pipette tips and the bottom of the wash stationduring the Dispense to Waste task. If you specify dynamic tip retraction, this is the starting distance. The labware definition must be accurate and the teachpoint must be precise in order for the system to position the tips at the correct distance from the well bottom. |
Dynamic tip retraction (mm/µL) | The rate at which to raise the pipette head during the Dispense to Waste task. Use dynamic tip retraction to prevent spills as the pipette tips displace the liquid. To move the pipettes: • At the same rate as the volume change. Calculate dynamic tip retraction (DTR) as follows: DTR = (well depth)/(well vol) = 1/A, where A is the cross-sectional area of a well with straight walls • Faster than the volume change. DTR > 1/A • Slower than the volume change. DTR < 1/A The starting and ending positions can be calculated as follows: (Vdispensed * DTR) + Distancewell bottom |
Well selection | The chimney locations at which the Dispense to Waste task occurs. Click in the parameter box, and then click the Browse button to select the locations in the Well Selection dialog box. Use this parameter only if the pipette head has fewer tips or cartridges than the number of locations in the wash station, or if you are in single-row or single-column mode. |
Perform tip touch on North/East side | The option to touch the tip on an outer side of the adjacent northeast chimney or wall in the wash stationafter performing the dispense. |
Tip touch retract distance (–20 to 50 mm) | The vertical distance for the pipette tips to rise before performing the tip touch. |
Tip touch horizontal offset (–9 to 5 mm) | The horizontal distance the tips move. The value is based on the well diameter specified by the labware definition. For example, if you set a value of: • 0, the tips move a horizontal distance equal to the well radius • > 0, the tips attempt to move past the well radius, which results in a more forceful tip touch • < 0, the tips move a distance less than the radius of the well, resulting in a lighter tip touch |
Example: Dispense into the wash station using a Bravo 96AM Head
Goal
After aspirating fluids up through mounted AssayMAP Bravo cartridges, remove the cartridges, dispense the contents of the Bravo 96AM Head syringes at the wash station, remount the cartridges for additional aspiration steps.
Implementation
In Bravo Diagnostics, set up the autofilling station (wash station) on a deck location. Associate the station with one or more pump modules.
In the protocol, add a configured labware for the wash station in addition to the other processes and configured labware required by the protocol. In the example shown, the configured reservoir is called Wash.
In the Bravo subprocess where the liquid-handling tasks are specified, a Dispense to Waste task is added after a series of aspiration tasks.
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In the Dispense to Waste Task Parameters area, the wash station location is selected so that the task is performed in this labware.
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Related information
For information about... | See... |
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Adding devices | • Bravo Platform User Guide |
Adding tasks in a protocol | |
Liquid classes | |
Pipette techniques | |
Aspirate task | |
Set Head mode task | |
Tips On task | |
Tips Off task | |
Microplate-handling tasks | |
Microplate-storage tasks | |
Scheduling tasks |