Tips On (Agilent Bravo)
Description
The Tips On (Agilent Bravo) (
) task presses disposable pipette tips onto the liquid-handling head. The task is not for use with fixed-tip pipette heads.
) task presses disposable pipette tips onto the liquid-handling head. The task is not for use with fixed-tip pipette heads.Task is available for... | Task is available in... |
|---|---|
Bravo Platform | Main Protocol, Bravo Subprocess |
Note: Options in Tips On and Tips Off tasks permit the tracking of pipette tip usage during and across protocol runs. In addition, an option in the Tips Off task allows you to reuse pipette tips for a portion of the protocol run.
Requirements
Tracking tips in serial dilution tasks
In addition to selecting the tracking option in the Tips On and Tips Off tasks, you must also turn on the tracking in the Serial Dilution Wizard. For more detailed information, see Serial Dilution (Agilent Bravo).
Tracking tips across different protocols
If you want to track pipette tip usage across different protocols that contain Bravo Subprocesses, make sure:
• The protocols reference the same device file.
• The tip boxes are at the same physical deck locations.
• In the software, the tip boxes are configured at the same locations across the protocols.
Note: When a set of tip boxes are designated as a process plate, tip usage is tracked during the protocol run. At the end of a run, the software resets the tip box to the original state. The software assumes that in each subsequent run, you will load tip boxes in the original state before the run.
Task parameters
After adding the Tips On task at the desired point in the protocol, set the following parameters in the Task Parameters area:
 |
Parameter | Description |
|---|---|
Location, plate | The labware involved in the Tips On task. |
Location, location | The location at which the Tips On task occurs. <auto-select> automatically places the labware at the first-available or appropriate location for the task. If accessories are installed on the deck, the software uses the accessory configuration information in Bravo Diagnostics to determine the correct location for the task. |
Allow automatic tracking of tip usage | The option to allow the software to track pipette tip usage during the protocol run or across different protocol runs. When you start the run, the software will determine the positions to use in the tip box. If you select the option in this task, you must also select the option in the Tips Off task. If you do not select this option, you must specify the positions to use in the tip box using the Well selection parameter. |
Well selection | The well positions to use for the Tips On task. This parameter is available only for manual tracking of pipette tips. |
Example: Press tips on before Bravo liquid-handling tasks
Goal
Make sure tips are pressed on before liquid-handling tasks start.
Implementation
In the liquid-handling subprocess, a Tips On task is added at the beginning. In the following example, because the protocol is run on a Bravo Platform, a Set Head Mode task must be added before the Tips On task.
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Related information
For information about... | See... |
|---|---|
Adding devices | • Device user guide |
Adding tasks in a protocol | |
Tips On task | |
Serial Dilution task | |
Set Head Mode task | |
Microplate-handling tasks | |
Microplate-storage tasks | |
Scheduling tasks |