Shake (Agilent Bravo)
Description
The Shake (Agilent Bravo) (
) task instructs the Orbital Shaking Station to shake.
) task instructs the Orbital Shaking Station to shake.Task is available for... | Task is available in... |
|---|---|
Bravo Platform | Main Protocol, Bravo Subprocess |
Requirements
To use the Shake task, you must first configure the Orbital Shaking Station in Bravo Diagnostics.
If you are using an Orbital Shaking Station that is connected to an Inheco STC or MTC Controller, use the Shake Plate task instead. See Shake Plate (Agilent Bravo).
Task parameters
After adding the Shake task at the desired point in the protocol, set the following parameters in the Task Parameters area:
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Parameter | Description |
|---|---|
Location, plate | The labware involved in the Shake task. |
Location, location | The location at which the Shake task occurs. <auto-select> automatically places the labware at the first-available or appropriate location for the task. If accessories are installed on the deck, the software uses the accessory configuration information in Bravo Diagnostics to determine the correct location for the task. |
Mode | The action of the task: • On. Turns on the Orbital Shaking Station. • Off. Turns off the Orbital Shaking Station. • Timed. Turns on the shaking timer. You must specify the length of time to shake. If you plan to time the shaking, add only one Shake task (the task turns on the shaking, and at the end of the time period, the shaking turns off automatically). If you are not timing the shaking, add two Shake tasks in the protocol (one to turn on the shaking and the other to turn off the shaking). |
RPM | The shake speed, in revolutions per minute. |
Direction | The direction to shake. Select one of the direction combinations: NWSE, NESW, NS, EW, NW/SE, NE/SW. |
Time for operation in Timed mode | The length of time, in seconds, you want to leave the shaking on. At the end of the period, the shaking will turn off. |
Allow concurrent operation | The option to permit the accessory to operate simultaneously with other tasks. For example, the Shake and Mix tasks can operate simultaneously.  To shake and mix concurrently, use only 96-well disposable-tip pipette heads in 96-well microplates. |
Example: Shake the destination microplate after adding reagents
Goal
Shake the destination microplate after adding reagents from two source microplates.
Implementation
In the liquid-handling subprocess, a Shake task is added after the second reagent is added. In the following example, a Shake task is added in a Bravo subprocess.
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In the Shake Task Parameters area, Destination is selected, because the goal is to shake the destination microplate.
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Related information
For information about... | See... |
|---|---|
Configured labware | |
Static labware | |
Startup Protocol | |
Adding devices | • Device user guide |
Adding tasks in a protocol | |
Microplate-handling tasks | |
Microplate-storage tasks | |
Scheduling tasks |