Location, plate

The labware involved in the Wash Tips task.

Location, location

The location at which the Wash Tips task occurs.

<auto-select> automatically places the labware at the first-available or appropriate location for the task. If accessories are installed on the deck, the software uses the accessory configuration information in Bravo Diagnostics to determine the correct location for the task.

Empty tips

The option to empty the entire contents of the pipette tips, including fluid and air. The Volume parameter is ignored if this option is selected.

Volume (µL)

The volume of liquid to be dispensed from each pipette tip.

Pre-aspirate volume (µL)

The volume of air to be drawn before the pipette tips enter the liquid.

Blowout volume (µL)

Specifies the volume of air to dispense after the main volume has been dispensed.

Typically, the blowout volume is the same as the pre-aspirate volume.

Liquid class

The liquid class associated with this liquid.

Mix cycles

The number of times you want to aspirate and dispense. Each cycle consists of one aspirate action and one dispense action.

Distance from well bottom (mm)

The distance between the end of the pipette tips and the well bottoms during the Wash Tips task.

Dynamic tip extension
(mm/(µL)

The rate at which the pipette head moves during the Wash Tips task. The software calculates the distance over which the tips will move without crashing.

Use dynamic tip extension to prevent spills as the pipette tips displace the liquid.

To move the tips:

The starting and ending positions can be calculated as follows:

(Vdispensed * DTE) + Dwell bottom

(Vaspirated * DTE) + Dwell bottom

Well selection

The wells at which the Wash Tips task occurs.

Click in the parameter box, and then click the Browse button to select the wells in the Well Selection dialog box.

Use this parameter only if the pipette head has fewer tips than the number of wells in the microplate, or if you are in single-row or single-column mode.

Perform tip touch

The option to touch the pipette tip on one or more sides of the well.

Which side to perform tip touch

The wall or walls for tip touch: North, South, East, West, North/South, West/East, West/East/South/North.

If you also select the Dispense to waste during wash option, the tip touch is performed on the northeast side only.

Tip touch retract distance

The vertical distance the pipette tips rise before touching the sides of the wells.

Tip touch horizontal offset

The horizontal distance the tips move. The value is based on the well diameter specified by the labware definition.

The value of the parameter determines the direction of movement:

Pump fill speed (%)

The speed, in percent of maximum speed, of liquid flow into the reservoir.

For the MicroWash Reservoir, this value should be high enough for the washing liquid to just bubble over the tops of the chimneys.

Pump empty speed (%)

The speed, in percent of maximum speed, of liquid flow out of the reservoir.

For the MicroWash Reservoir, this value should be slightly higher than that of the inflow pump to prevent an overflow.

Turn pumps off during dispense

The option to turn off the pumps while dispensing liquid during this task.

Dispense to waste during wash

The option to move the tips by a specified offset (defined in the Labware Editor) and dispense used fluid outside of the reservoir chimney.

This option applies only to reservoirs that have chimneys.

Dispense to waste at height (mm)

The height at which the dispense action occurs.

For example, during the dispense action, the tips move up to clear the chimneys, move the offset distance, and then lower to the distance you specified. If you want the lower the tips by 10 mm, specify –10 mm.